Study of Serum Hepcidin Level Variations in Sickle Cell Disease: A Meta-analysis Study
Chandrakant G Kamble, Padhyegurjar Shekhar, Prashant J Hisalkar, Powar D Jagdish Santosh E Bidwe, Bhausaheb V Jagadale
INTRODUCTION: Serum hepcidin has been observed in sickle cell disease (SCD). The biological mechanism of the synthesis and regulation of hepcidin remain unclear. The aim of meta-analysis is to provide an overview of the serum hepcidin level changes and to discuss the significance of serum hepcidin level in SCD. MATERIALS AND METHODS: The meta-analysis and a systematic review is to determine serum hepcidin level changes during SCD and was conducted under the Preferred Reporting Items for Systematic reviews and Meta-analysis (PRISMA) guidelines. Without language restrictions, the articles were identified through BioMed, EMBASE, PubMedÂ® (US National Library of Medicine, USA), bloodjournal.org, PLoS ONE, Web of Science, LILACS (Latin American and Caribbean Health Sciences Literature), ISI Web of Science, Chemical abstract services (CAS), Europe PMC, BIOSIS Previews, Elsevier Properties SA, USA. The studies had been published before November 30, 2017. RESULTS: We identified 14 prospective studies out of 351 articles and included for the meta-analysis. From the final selected articles, the mean, standard deviation, sample size of cases vs controls were compared and calculated by Statistical Package for the Social Sciences (SPSS) latest software version 24. From 14, nine articles were statistically highly significant, and remaining five articles were statistically not significant; p < 0.005 was considered to be statistically significant. The combined estimation of the meta-analysis study is not significant, since p = 0.32, calculated â€œtâ€ = â€“0.99, and degree of freedom is 979. CONCLUSION: The study concluded that there is no significant difference between mean serum hepcidin of cases and healthy controls. However, serum hepcidin levels might be increased in SCD, but not after the frequent blood transfusion SCD patients, so our hypothesis is not strongly accepted and does not reach conclusive positions. Hence, we will study further the same task by separating the serum hepcidin level in SCD patients before and after frequent blood transfusion.
Meta-analysis, Serum hepcidin level, Sickle cell disease.