Study of Serum Zinc, Vitamin A and Alkaline Phosphatase Status in Nyctalopia Patients
Chandrakant G Kamble, Girish Shakuntal, Jagadish D Powar, Santosh E Bidwe, Bhausaheb V Jagdale
BACKGROUND: Nyctalopia is a condition in which the person is unable to see in the dim light/dark since the dark adaptation time increases. The condition is due to deficiency of vitamin A; it is not an only one factor responsible for nyctalopia, along with that Zinc is also the metal ion enzyme responsible for the development of nyctalopia. The zinc ion is responsible for mobilization of stored form of vitamin A (retinyl palmitate to retinol) and results into the alternations of retinol processing in retinal pigment epithelial cells in the visual cycle. Hence the objectives of the present study were the estimation of serum vitamin A, Zn++ and serun alkaline phosphatase (ALP) (zinc-dependent enzyme) had been added to see the effect in the present study. METHODS: The total 30 number of nyctalopia patients and 30 numbers of healthy subjects were selected for the present study. SAMPLE COLLECTION AND PROCESSING: The fasting blood samples were collected in the plain bulb, and then it was used for laboratory processing like centrifugation and estimations. Serum vitamin A was estimated by Bessey method. Serum zinc was estimated by colorimetric (kit) method and serum ALP were estimated by the king and king method. RESULTS: In this study, the estimated serum vitamin A levels in the test was found to be mean Â± SD = 13.69 Â± 2.82 and controls were 29.04 Â± 1.97. The calculated p < 0.0000 so it was statistiÂ¬cally very highly significant. Serum Zn++, test was 18.87 Â± 2.47 and controls were found to be 61.86 Â± 1.74. The calculated p < 0.0000, so it was statistically very highly significant. But in case of the calculated serum ALP, it had been found to be nearly the same (very trace increase) in test and control groups of the patients, i.e., 545.97 Â± 39.79 and 546.20 Â± 39.77, and the p value was found to be p < 0.98. So it was not found significant. CONCLUSION: In this study, the serum vitamin A and Zn++ had been found to be decreased below the normal levels in nyctaÂ¬lopia patients. But the serum ALP levels had been found to be nearly the same (very trace increase) in test and control group patients.